9EGW

HOIL-1 RING2 domain bound to ubiquitin-maltose (maltose not modelled)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	I 41 2 2
Unit cell lengths	96.063, 96.063, 187.434
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.860 - 1.780
R-factor	0.1657
Rwork	0.165
R-free	0.18210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.211
Data reduction software	XDS (Nov 1, 2016 BUILT=20161205)
Data scaling software	Aimless (0.7.8)
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.860	1.820
High resolution limit [Å]	1.780	1.780
Rmerge	0.115	1.449
Rmeas	0.119	1.508
Rpim	0.032	0.414
Total number of observations	571589	30180
Number of reflections	42270	2337
<I/σ(I)>	12.9	1.3
Completeness [%]	99.9
Redundancy	13.5	12.9
CC(1/2)	0.999	0.806

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.02 M MgCl2, 0.05 M MES pH6 and 1.8 M LiSO4