9EGN

Crystal structure of the human Cavin1 HR1 domain bound to nanobody B7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 63
Unit cell lengths	58.897, 58.897, 113.122
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.880 - 1.570
R-factor	0.1738
Rwork	0.173
R-free	0.19040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.372
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.000	1.600
High resolution limit [Å]	1.570	1.570
Rpim	0.100	0.107
Number of reflections	31084	1492
<I/σ(I)>	28.3	4
Completeness [%]	99.7
Redundancy	20
CC(1/2)	1.000	0.960

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.65 M potassium thiocyanate, 19.5% PEG 2000 MME