9EG6
Crystal structure of the human Cavin1 HR1 HT/II mutant domain bound to nanobody B7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.315, 59.243, 133.815 |
| Unit cell angles | 90.00, 97.48, 90.00 |
Refinement procedure
| Resolution | 44.230 - 3.200 |
| R-factor | 0.2597 |
| Rwork | 0.258 |
| R-free | 0.28670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.870 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rpim | 0.116 | 0.502 |
| Number of reflections | 13349 | 2402 |
| <I/σ(I)> | 6.6 | 2.6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.6 | 6.4 |
| CC(1/2) | 0.991 | 0.878 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M potassium thiocyanate, 20% PEG 3350 and 0.2 M potassium citrate, 20% PEG 3350 |
