9EFN
Crystal structure of Saccharomyces cerevisiae cross-linked Sfh1 (K197C, F233C) mutant in complex with phosphatidylethanolamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-13 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9202 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.430, 72.010, 114.680 |
| Unit cell angles | 90.00, 93.95, 90.00 |
Refinement procedure
| Resolution | 46.460 - 1.750 |
| R-factor | 0.2426 |
| Rwork | 0.241 |
| R-free | 0.27890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.963 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.310 | 1.813 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmeas | 0.142 | 0.990 |
| Number of reflections | 80061 | 7936 |
| <I/σ(I)> | 7.23 | |
| Completeness [%] | 98.8 | 98.62 |
| Redundancy | 4 | 4.2 |
| CC(1/2) | 0.993 | 0.681 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 296 | 0.1 M ammonium sulfate, 0.1 M Bis-Tris, pH 5.5 |
