9EFL
Structure of a GH16_17 carrageenase from a metagenomic dataset.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-08-09 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 56.719, 69.632, 109.059 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.670 - 2.000 |
| R-factor | 0.177 |
| Rwork | 0.175 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.896 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmeas | 0.162 | 1.140 |
| Rpim | 0.071 | 0.493 |
| Number of reflections | 29675 | 2176 |
| <I/σ(I)> | 7.5 | 1.6 |
| Completeness [%] | 99.5 | 99.6 |
| Redundancy | 4.7 | 4.9 |
| CC(1/2) | 0.994 | 0.586 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 291 | 0.2 M Sodium chloride, 0.1 M Na2HPO4:citric acid, 20% PEG 8000 |
