9EF3

Human Hsp27 alpha-crystallin domain (84-171) in complex with a peptide mimic of its C-terminus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.920
Spacegroup name	P 43 21 2
Unit cell lengths	56.060, 56.060, 166.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.690 - 2.250
R-factor	0.2332
Rwork	0.232
R-free	0.25380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.569
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.690	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.076	1.698
Rmeas	0.078	1.732
Rpim	0.015	0.335
Number of reflections	13304	1287
<I/σ(I)>	34.2	2.3
Completeness [%]	99.9	100
Redundancy	25	26.1
CC(1/2)	1.000	0.819

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	0.2M ammonium sulfate, 0.1M sodium cacodylate trihydrate, pH 6.5, 22-24% w/v PEG 8000