9EE3
HIV CA - FG peptide (14 mM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-16 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.920099 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 135.589, 135.864, 206.547 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.010 - 3.100 |
| R-factor | 0.2295 |
| Rwork | 0.228 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.398 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.010 | 3.290 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 67035 | 10777 |
| <I/σ(I)> | 6.44 | 2.12 |
| Completeness [%] | 96.0 | |
| Redundancy | 3.41 | 3.3 |
| CC(1/2) | 0.990 | 0.730 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100 mM Hepes, pH 7.4, 10% PEG 4,000 |
