9EE2
HIV CA - FSFG peptide (14 mM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-23 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.920119 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 136.408, 136.447, 208.854 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.470 - 2.990 |
| R-factor | 0.2338 |
| Rwork | 0.233 |
| R-free | 0.25340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.387 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.470 | 3.170 |
| High resolution limit [Å] | 2.990 | 2.990 |
| Number of reflections | 78145 | 12309 |
| <I/σ(I)> | 9.64 | 1.59 |
| Completeness [%] | 98.8 | 98.8 |
| Redundancy | 5.71 | 5.79 |
| CC(1/2) | 0.997 | 0.506 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100 mM Hepes, pH 7.4, 10% PEG 4,000 |
