9EE1
HIV CA - GLFG peptide (43 mM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.920105 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 135.868, 136.254, 206.677 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.200 - 3.000 |
| R-factor | 0.2252 |
| Rwork | 0.223 |
| R-free | 0.26090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.395 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.200 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 77143 | 12244 |
| <I/σ(I)> | 8.45 | 1.75 |
| Completeness [%] | 99.5 | 99.3 |
| Redundancy | 4.23 | 4.31 |
| CC(1/2) | 0.995 | 0.649 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100 mM Hepes, pH 7.4, 10% PEG 4,000 |
