9EE0
HIV CA - GLFG peptide (9 mM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979338 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 135.064, 135.219, 205.749 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.900 - 3.100 |
| R-factor | 0.2304 |
| Rwork | 0.229 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.414 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.900 | 3.280 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.703 | |
| Number of reflections | 66167 | 10573 |
| <I/σ(I)> | 7.46 | 1.75 |
| Completeness [%] | 95.4 | 96.2 |
| Redundancy | 3.96 | 3.72 |
| CC(1/2) | 0.993 | 0.650 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100 mM Hepes, pH 7.4 10% PEG 4,000 |
