9EDZ
HIV CA - GLFG peptide (4 mM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-23 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.920119 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 136.547, 136.778, 208.195 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.700 - 3.000 |
| R-factor | 0.238 |
| Rwork | 0.236 |
| R-free | 0.26720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.400 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.700 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 77790 | 12377 |
| <I/σ(I)> | 8.51 | 1.91 |
| Completeness [%] | 98.5 | 98.6 |
| Redundancy | 4.99 | 5.02 |
| CC(1/2) | 0.995 | 0.614 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100 mM Hepes, pH 7.4 10% PEG 4,000 |
