9EDF

Streptavidin-S112C-L124F bound to Cu(II)dpea cofactor

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-19
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0
Spacegroup name	I 41 2 2
Unit cell lengths	57.620, 57.620, 183.759
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.980 - 1.570
R-factor	0.1626
Rwork	0.162
R-free	0.18350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.370
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.980	1.590
High resolution limit [Å]	1.570	1.570
Rmerge	0.079	0.139
Rmeas		0.151
Rpim	0.042	0.058
Number of reflections	21503	1055
<I/σ(I)>	15.4
Completeness [%]	97.0
Redundancy	6.5
CC(1/2)	0.997	0.988

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	298	ammonium sulfate, sodium acetate