9ECT

Crystal Structure of the Gemella haemolysans Immunoglobulin A1 Protease Trypsin-Like Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-11
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.521
Spacegroup name	P 1 21 1
Unit cell lengths	47.271, 58.550, 76.368
Unit cell angles	90.00, 103.66, 90.00

Refinement procedure

Resolution	74.210 - 1.750
R-factor	0.1961
Rwork	0.194
R-free	0.24210
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	MR-Rosetta
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.210	1.813
High resolution limit [Å]	1.750	1.750
Rmerge	0.205	0.621
Rmeas	0.224
Number of reflections	40706	4006
<I/σ(I)>	2.6
Completeness [%]	99.3	98.86
Redundancy	5.7
CC(1/2)	0.984	0.835

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2M KNO3, 25% (w/v) PEG3350