9ECI

Crystal structure of a humanized 5E5 antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	292.15
Detector technology	PIXEL
Collection date	2020-01-23
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	102.237, 75.343, 69.651
Unit cell angles	90.00, 106.28, 90.00

Refinement procedure

Resolution	33.430 - 1.570
R-factor	0.1571
Rwork	0.156
R-free	0.18310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.883
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.600
High resolution limit [Å]	1.570	1.570
Number of reflections	70587	3481
<I/σ(I)>	17.4
Completeness [%]	99.9
Redundancy	6.6
CC(1/2)	0.970	0.895

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	292.15	PEG 4000, sodium acetate, Tris