9EC1

The Structure of Human Dystrophin Spectrin Repeat 24

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920085
Spacegroup name	P 65 2 2
Unit cell lengths	106.003, 106.003, 614.847
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.480 - 2.140
R-factor	0.2187
Rwork	0.217
R-free	0.25450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.668
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.480	2.360
High resolution limit [Å]	2.140	2.140
Rmerge	0.196	1.643
Rmeas	0.202	1.643
Rpim	0.046	0.448
Number of reflections	71466	3574
<I/σ(I)>	13.1
Completeness [%]	95.8	78.9
Redundancy	19	14.2
CC(1/2)	0.999	0.581

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1 M Sodium citrate tribasic dihydrate, pH 5.6, 0.5 M Ammonium sulfate , 1 M Lithium sulfate monohydrate