9EB3

Chicken YF1.7*1 presenting N-myristoylated glycine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	I 1 2 1
Unit cell lengths	52.940, 54.940, 136.244
Unit cell angles	90.00, 98.50, 90.00

Refinement procedure

Resolution	34.770 - 2.300
R-factor	0.1828
Rwork	0.180
R-free	0.23570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.952
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.0.32)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.770	2.382
High resolution limit [Å]	2.300	2.300
Rmerge	0.060	0.310
Rmeas	0.084	0.440
Rpim	0.060	0.310
Number of reflections	17376	1706
<I/σ(I)>	8.3	2.15
Completeness [%]	99.9	100
Redundancy	2	2
CC(1/2)	0.994	0.796

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	25% w/v PEG 3350, 200 mM lithium sulfate, 100 mM bis-tris propane pH 5.5