9EB2

Chicken YF1*7.1 molecule presenting an endogenous N-myristoylated amino acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-02
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	53.840, 61.340, 134.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.340 - 2.000
R-factor	0.1646
Rwork	0.162
R-free	0.20940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.851
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.0.32)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.340	2.071
High resolution limit [Å]	2.000	2.000
Rmerge		0.112
Rmeas	0.035	0.158
Rpim	0.025	0.112
Number of reflections	30861	3014
<I/σ(I)>	14.04	5.55
Completeness [%]	99.9	99.44
Redundancy	2	2
CC(1/2)	0.999	0.955

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	16% w/v PEG3350, 200 mM ammonium dihydrogen phosphate