9EAX
CRYSTAL STRUCTURE OF THE FMN-BINDING DOMAIN OF HUMAN CYTOCHROME P450 REDUCTASE (MUTANT V164M)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.188, 50.474, 47.110 |
| Unit cell angles | 90.00, 106.06, 90.00 |
Refinement procedure
| Resolution | 37.660 - 1.350 |
| R-factor | 0.1597 |
| Rwork | 0.159 |
| R-free | 0.17900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.077 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.660 | 1.420 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmeas | 0.266 | |
| Number of reflections | 38471 | 5559 |
| <I/σ(I)> | 7.1 | 3.1 |
| Completeness [%] | 98.9 | 98.1 |
| Redundancy | 6.3 | 5.9 |
| CC(1/2) | 0.930 | 0.100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 0.1 M HEPES pH 7.0, 0.3 M Calcium Chloride, 21% PEG 400 |
