9E8E

Hybrid G-quadruplex from Tetrahymena thermophila telomeric sequence in complex with TrisQO Form 2

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	183
Detector technology	PIXEL
Collection date	2024-07-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97934
Spacegroup name	P 61 2 2
Unit cell lengths	55.778, 55.778, 110.523
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.290 - 1.650
R-factor	0.178
Rwork	0.176
R-free	0.21640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.847
Data reduction software	autoPROC
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.300	1.680
High resolution limit [Å]	1.650	1.650
Number of reflections	12801	612
<I/σ(I)>	10.6
Completeness [%]	100.0
Redundancy	20.2
CC(1/2)	1.000	0.363

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	potassium chloride, calcium chloride dihydrate, HEPES sodium pH 7.0, PEG 400