9E7C

TRK-fused Gene (TFG) PB1 Domain D60A/D62R Variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	203
Detector technology	CCD
Collection date	2013-12-01
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	C 1 2 1
Unit cell lengths	107.018, 32.488, 29.246
Unit cell angles	90.00, 104.80, 90.00

Refinement procedure

Resolution	51.730 - 1.906
R-factor	0.21555
Rwork	0.214
R-free	0.25406
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.342
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	51.730	50.000	1.940
High resolution limit [Å]	1.906	5.180	1.910
Rmerge		0.031	0.536
Rmeas	0.076	0.033	0.582
Rpim	0.028	0.013	0.224
Number of reflections	7690	413	342
<I/σ(I)>	7.9
Completeness [%]	99.4	99.8	94
Redundancy	7.2	7	6.1
CC(1/2)		0.999	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	0.1M Tris-HCl pH 8.5, 8% PEG 8000