9E6T

BCL11A ZF4-6 in Complex with a DNA Sequence Observed in the Human Globin Locus Containing Motif TGCCCA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-27
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92010
Spacegroup name	P 41 21 2
Unit cell lengths	59.230, 59.230, 247.124
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.820 - 2.780
R-factor	0.2479
Rwork	0.246
R-free	0.28060
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.003
RMSD bond angle	0.490
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.820	2.820
High resolution limit [Å]	2.780	2.780
Rpim	0.065
Number of reflections	20789	1057
<I/σ(I)>	10.7
Completeness [%]	100.0	100
Redundancy	25.8	27.3
CC(1/2)	0.998	0.429

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	292	15 % w/v PEG 1000, 30 % v/v PEG 300, 0.1 M Bis-Tris Propane 7.0