9E6R
BCL11A ZF4-6 in Complex with a DNA Sequence Observed in the Human Globin Locus Containing Motif TGACCA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-04 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.92010 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 61.328, 61.328, 244.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.930 - 2.090 |
| R-factor | 0.2059 |
| Rwork | 0.204 |
| R-free | 0.23830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.739 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.930 | 2.130 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.116 | |
| Rpim | 0.048 | 0.964 |
| Number of reflections | 52590 | 2838 |
| <I/σ(I)> | 9.8 | 0.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7 | 7.3 |
| CC(1/2) | 0.997 | 0.350 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 292 | 24 % v/v Ethanol, 0.1 M HEPES 7.8, 40 mM Magnesium chloride hexahydrate |
