9E69
Antibody 5E10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2019-12-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 |
| Unit cell lengths | 93.969, 93.969, 107.667 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.060 - 2.630 |
| R-factor | 0.2195 |
| Rwork | 0.218 |
| R-free | 0.24220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.816 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.727 |
| High resolution limit [Å] | 2.630 | 2.633 |
| Rmerge | 0.068 | |
| Rpim | 0.049 | 0.684 |
| Number of reflections | 30869 | 2965 |
| <I/σ(I)> | 31.6 | 1.15 |
| Completeness [%] | 97.7 | 94.5 |
| Redundancy | 5.1 | 5.1 |
| CC(1/2) | 0.999 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | JCSG1 A8 (#8) 0.2 M sodium acetate 20% PEG3350 |
