9E5B
Crystal structure of Cotonvirus japonicum vIF4E 1-236 in complex with m7Gppp(2'OMeA)pU
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2024-04-07 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | .92010 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 143.918, 143.918, 74.954 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.570 - 2.260 |
| R-factor | 0.2428 |
| Rwork | 0.241 |
| R-free | 0.27340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.588 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.570 | 2.380 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rpim | 0.039 | 1.193 |
| Number of reflections | 22032 | 3135 |
| <I/σ(I)> | 12.2 | 0.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 24.5 | 25.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 10% PEG 6000, 2.0 M NaCl |
