9E5A
Crystal structure of Acanthamoeba polyphaga mimivirus (APMV) vIF4G 53-312
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2023-12-10 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.654, 91.317, 58.529 |
| Unit cell angles | 90.00, 117.54, 90.00 |
Refinement procedure
| Resolution | 49.350 - 1.460 |
| R-factor | 0.1769 |
| Rwork | 0.176 |
| R-free | 0.19640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.583 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.350 | 1.490 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rpim | 0.037 | 0.856 |
| Number of reflections | 86228 | 4214 |
| <I/σ(I)> | 10.4 | 0.8 |
| Completeness [%] | 96.7 | 95.6 |
| Redundancy | 7.3 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M NaSCN and 20% PEG 3350 |
