9E44
Crystal Structure of FosA from Pseudomonas aeruginosa with Manganese and 2-Phosphonopropionic acid
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU PhotonJet-R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-07 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.5406 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.835, 64.535, 74.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.230 - 1.800 |
| R-factor | 0.1818 |
| Rwork | 0.179 |
| R-free | 0.21740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.820 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.230 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rpim | 0.035 | 0.327 |
| Number of reflections | 25027 | 1769 |
| <I/σ(I)> | 15.22 | |
| Completeness [%] | 99.7 | |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 76 mM K2HPO4 (pH 7.0), 40% PEP629, 1 mM MnCl2, and 40 mM KCl |
