9E42

RTA-RUNT-192 complex

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	38.917, 56.944, 124.964
Unit cell angles	90.00, 93.64, 90.00

Refinement procedure

Resolution	42.050 - 1.800
R-factor	0.2201
Rwork	0.219
R-free	0.24610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.709
Data reduction software	RAPD
Data scaling software	RAPD
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.120	0.920
Number of reflections	333976	17035
<I/σ(I)>	10.7
Completeness [%]	99.5
Redundancy	6.6
CC(1/2)	0.990	0.610

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	170 mM diammonium hydrogen phosphate