9DZX

MHV spike tail heptapeptide complexed with coatomer alpha-WD40 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92011
Spacegroup name	P 21 21 21
Unit cell lengths	38.118, 75.126, 129.335
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.990 - 1.660
R-factor	0.1779
Rwork	0.176
R-free	0.20750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.950
Data reduction software	HKL-3000
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.990	1.660
High resolution limit [Å]	1.660	1.660
Rmerge	0.037	0.120
Rmeas	0.135	0.055
Rpim	0.046	0.026
Number of reflections	44353	44487
<I/σ(I)>	9.6	9.6
Completeness [%]	99.2	99.5
Redundancy	2
CC(1/2)	0.998	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295.15	0.1 M HEPES pH 7.5, 10% PEG 8000, 8% Ethylene Glycol