9DZS

Acanthamoeba Polyphaga Mimivirus R699

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	110
Detector technology	PIXEL
Collection date	2022-07-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	118.041, 71.481, 72.626
Unit cell angles	90.00, 123.18, 90.00

Refinement procedure

Resolution	57.980 - 1.750
Rwork	0.171
R-free	0.19530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.695
Data reduction software	xia2 (3.8.0)
Data scaling software	Aimless (0.7.8)
Phasing software	PHENIX (1.20.1)
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.790	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.083	0.491
Number of reflections	349711	18358
<I/σ(I)>	10.9	2.7
Completeness [%]	99.3	98.5
Redundancy	6.9	6.6
CC(1/2)	0.998	0.921

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Protein/cofactor mix: 12mg/ml R699, 10mM MnCl2, 10mM UDP glucose, 50% glycerol. Mother liquor: 0.18 M Ammonium chloride, 18% (w/v) PEG 3350, 4% v/v 1-Propanol. The ratio of mix and mother liquor was at 1:1.