9DZD

PvRBP2b N-terminal domain stabilised mutant WHT2484

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953647
Spacegroup name	P 41 21 2
Unit cell lengths	46.423, 46.423, 361.005
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.040 - 1.850
R-factor	0.1909
Rwork	0.189
R-free	0.22160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.817
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.040	1.890
High resolution limit [Å]	1.850	1.850
Rmerge	0.078	1.513
Rmeas	0.082
Rpim	0.023	0.423
Total number of observations	450878
Number of reflections	35632	2099
<I/σ(I)>	19.5
Completeness [%]	100.0
Redundancy	12.7	13.4
CC(1/2)	1.000	0.792

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	10 % Propan-2-ol 10 % PEG MME 5000 0.1 M Na Cacodylate pH 6