9DZC
PvRBP2b N-terminal domain stabilised mutant WHT2483
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.953647 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.237, 92.828, 118.205 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.050 - 2.400 |
| R-factor | 0.2004 |
| Rwork | 0.198 |
| R-free | 0.24510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.441 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.380 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 1.066 | |
| Rpim | 0.425 | |
| Number of reflections | 34857 | 3560 |
| <I/σ(I)> | 15.7 | 2.5 |
| Completeness [%] | 99.8 | 98 |
| Redundancy | 13.5 | 13.6 |
| CC(1/2) | 0.999 | 0.806 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 5 % MPD 10 % PEG 6000 0.1 M HEPES pH 7.5 |
