9DZA
Photoactivation in Bacteriophytochrome, high resolution cryo structure in the dark.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2020-09-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.330, 81.690, 83.490 |
| Unit cell angles | 90.00, 107.07, 90.00 |
Refinement procedure
| Resolution | 56.680 - 1.400 |
| R-factor | 0.1979 |
| Rwork | 0.195 |
| R-free | 0.24460 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.882 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.000 | 1.430 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 196338 | 32602 |
| <I/σ(I)> | 4.4 | |
| Completeness [%] | 93.0 | 93 |
| Redundancy | 3.5 | 3.4 |
| CC(1/2) | 0.980 | 0.200 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | Hampton Crystal Screen, Cryo, HR2-122, #9 plus 3 % benzamidine HCl, 1:1 with protein, 30 mg/ml. |
