9DZ7
Structure of ALAS bound to glycine from S. cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-08 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.920105 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.672, 112.518, 117.388 |
| Unit cell angles | 115.76, 98.31, 91.49 |
Refinement procedure
| Resolution | 48.910 - 2.487 |
| R-factor | 0.1698 |
| Rwork | 0.168 |
| R-free | 0.20740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.286 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.912 | 2.630 |
| High resolution limit [Å] | 2.487 | 2.490 |
| Rmeas | 0.177 | 0.892 |
| Number of reflections | 93703 | 14980 |
| <I/σ(I)> | 6.27 | 1.43 |
| Completeness [%] | 98.4 | 97.9 |
| Redundancy | 3.6 | 3.4 |
| CC(1/2) | 0.988 | 0.577 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.15 M magnesium chloride, 0.1 M HEPES, pH 7.5, 25% v/v PEG3350 |
