9DYR

Crystal Structure of Rubisco in complex with CABP from Methanococcoides burtonii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-16
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.00002
Spacegroup name	P 21 21 21
Unit cell lengths	80.844, 247.856, 266.093
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.180 - 2.660
R-factor	0.2529
Rwork	0.253
R-free	0.27710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.885
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.180	2.690
High resolution limit [Å]	2.660	2.660
Number of reflections	1145457	57131
<I/σ(I)>	9.6
Completeness [%]	99.5
Redundancy	7.4
CC(1/2)	0.996	0.759

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M ammonium sulfate, 20 % PEG 3,350