9DYQ

Crystal structure of human dysferlin C2G domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 2
Unit cell lengths	83.942, 109.760, 59.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.040 - 2.050
R-factor	0.2466
Rwork	0.245
R-free	0.27260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.170
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.040	2.123
High resolution limit [Å]	2.050	2.050
Rmerge	0.161	3.364
Rmeas	0.168	3.523
Rpim	0.049	1.021
Number of reflections	35165	3423
<I/σ(I)>	6.62	0.69
Completeness [%]	97.5	97.29
Redundancy	12.1	12.3
CC(1/2)	0.995	0.763

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	293	0.14 M calcium chloride, 10% 2-propanol, 15% glycerol, 0.07 M sodium acetate trihydrate