9DYP

Crystal Structure of Rubisco from Methanococcoides burtonii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-11
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97741
Spacegroup name	P 1 21 1
Unit cell lengths	86.033, 102.823, 97.732
Unit cell angles	90.00, 90.14, 90.00

Refinement procedure

Resolution	45.500 - 1.500
R-factor	0.1622
Rwork	0.162
R-free	0.18570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.931
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.500	1.540
High resolution limit [Å]	1.500	1.500
Number of reflections	271048	19315
<I/σ(I)>	7.1
Completeness [%]	99.9
Redundancy	3.8
CC(1/2)	0.996	0.320

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.17 M sodium acetate, 0.085 M Tris-HCl pH 8.5, 25.5 % PEG 4,000, 15 % glycerol