9DYA

CHIP-TPR in complex with the Hsp70 tail

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000000
Spacegroup name	C 2 2 21
Unit cell lengths	46.899, 74.363, 77.634
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.670 - 1.890
R-factor	0.1938
Rwork	0.190
R-free	0.23370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.156
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21rc1_5127)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.670	1.980
High resolution limit [Å]	1.890	1.890
Rmerge	0.170	1.014
Rpim	0.101	0.655
Number of reflections	20723	2406
<I/σ(I)>	7.05	0.99
Completeness [%]	98.9	91.46
Redundancy	3.8	3.2
CC(1/2)	0.988	0.442

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	0.2 M lithium sulfate, 0.1 M Tris-HCl, 30% (w/v) PEG 4000