9DX6

Crystal structure of Plasmodium vivax (Palo Alto) PvAMA1 in complex with human Fab 826827

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953724563122
Spacegroup name	C 1 2 1
Unit cell lengths	187.237, 54.461, 104.130
Unit cell angles	90.00, 97.20, 90.00

Refinement procedure

Resolution	47.760 - 2.400
R-factor	0.1936
Rwork	0.192
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.668
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.760	7.120	2.540
High resolution limit [Å]	2.400	5.060	2.400
Rmerge	0.166	0.079	1.086
Rmeas	0.180	0.086	1.176
Number of reflections	41378	2877	6614
<I/σ(I)>	9.53
Completeness [%]	99.9
Redundancy	7
CC(1/2)	0.995	0.996	0.642

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	22% PEG Smear Broad, 0.1 M Tris pH 8.5, 0.3 M Glycyl-glycyl-glycine