9DWO
Crystal Structure of C4-Dicarboxylate-Binding Protein (PA0884) of Tripartite ATP-independent Periplasmic Transporter Family from Pseudomonas aeruginosa PAO1 in Complex with L-Malate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-19 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.12723 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.678, 66.953, 87.688 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.840 - 1.500 |
| R-factor | 0.17648 |
| Rwork | 0.175 |
| R-free | 0.21209 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.488 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.140 | |
| Rmeas | 0.146 | |
| Rpim | 0.040 | 0.356 |
| Number of reflections | 44360 | 2172 |
| <I/σ(I)> | 18.7 | 2.4 |
| Completeness [%] | 98.6 | 98.1 |
| Redundancy | 12.5 | 12.9 |
| CC(1/2) | 0.998 | 0.607 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 292 | Protein: 8.5 mg/ml, 0.15M Sodium chloride, 0.02M Tris-HCl (pH 8.0), 2mM L-Malate; Screen: PACT (D6), 0.1M MMT buffer pH 9.0, 25% (w/v) PEG 1500; Cryo: Reservoir |
