9DVG
Crystal Structure of a DARPin Fused to the 1TEL Crystallization Chaperone via a Direct Helical Fusion in a 2-Fold Crystal Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 65 |
| Unit cell lengths | 101.461, 101.461, 48.077 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.730 - 3.540 |
| R-factor | 0.2954 |
| Rwork | 0.295 |
| R-free | 0.30260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.665 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.730 | 3.670 |
| High resolution limit [Å] | 3.540 | 3.540 |
| Rmerge | 0.192 | 2.487 |
| Rmeas | 0.197 | 2.562 |
| Rpim | 0.043 | 0.609 |
| Number of reflections | 3560 | 359 |
| <I/σ(I)> | 8.12 | 1.86 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 20.6 | 17.3 |
| CC(1/2) | 0.999 | 0.407 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 1.5 M Ammonium Phosphate Dibasic, 0.1 M Tris |
