9DSK
Crystal structure of the cow antibody 115
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 2.0.1 |
| Synchrotron site | ALS |
| Beamline | 2.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0358 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.042, 71.738, 61.990 |
| Unit cell angles | 90.00, 108.45, 90.00 |
Refinement procedure
| Resolution | 46.640 - 1.700 |
| R-factor | 0.1714 |
| Rwork | 0.169 |
| R-free | 0.22790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.819 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.640 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.051 | 1.296 |
| Rmeas | 0.062 | 1.566 |
| Rpim | 0.033 | 0.867 |
| Number of reflections | 49417 | 2519 |
| <I/σ(I)> | 10.1 | 0.9 |
| Completeness [%] | 99.7 | 97.1 |
| Redundancy | 3.4 | 3.1 |
| CC(1/2) | 0.991 | 0.427 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M HEPES sodium pH 7.5, 10% v/v 2-propanol, 20% w/v polyethylene glycol 4,000 |
