9DSJ

Crystal structure of the cow antibody 105

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-12
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 2
Unit cell lengths	70.780, 154.705, 54.126
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.680 - 1.650
R-factor	0.1678
Rwork	0.167
R-free	0.21110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.809
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.680	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.077	3.031
Rmeas	0.080	3.160
Rpim	0.022	0.877
Number of reflections	72405	3410
<I/σ(I)>	16.2	1.2
Completeness [%]	99.7	96.7
Redundancy	13.5	12.6
CC(1/2)	0.999	0.421

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.12 M alcohol, 0.1M buffer system 3 pH 8.5, 30% precipitant mix 1