9DSI

Crystal structure of the cow antibody 99

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-12
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	66.400, 71.097, 151.044
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.760 - 2.800
R-factor	0.2328
Rwork	0.229
R-free	0.26680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.511
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.760	2.950
High resolution limit [Å]	2.790	2.790
Rmerge	0.276	2.878
Rmeas	0.286	2.988
Rpim	0.077	0.796
Number of reflections	18350	2591
<I/σ(I)>	8.9	1.4
Completeness [%]	99.7	98.7
Redundancy	13.5	13.7
CC(1/2)	0.994	0.626

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M HEPES 7.0, 30 % v/v Jeffamine ED-2003