9DSH

Crystal structure of the cow antibody P7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-08-10
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	62.442, 67.707, 109.135
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.130 - 1.700
R-factor	0.1824
Rwork	0.181
R-free	0.22270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.995
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.380	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.089	1.543
Rmeas	0.092	1.609
Rpim	0.025	0.451
Number of reflections	51716	2675
<I/σ(I)>	14.9	1.7
Completeness [%]	99.9	99.3
Redundancy	13.6	12.4
CC(1/2)	0.999	0.774

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2 M sodium bromide, 20% w/v polyethylene glycol 3,350