9DSG
Crystal structure of the SARS-CoV-2 RBD in complex with the cow antibody P2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 2.0.1 |
| Synchrotron site | ALS |
| Beamline | 2.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.035 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.971, 68.147, 82.656 |
| Unit cell angles | 90.00, 90.73, 90.00 |
Refinement procedure
| Resolution | 41.320 - 2.400 |
| R-factor | 0.2247 |
| Rwork | 0.222 |
| R-free | 0.26310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.542 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.400 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.116 | 1.356 |
| Rmeas | 0.139 | 1.602 |
| Rpim | 0.075 | 0.847 |
| Number of reflections | 28910 | 2993 |
| <I/σ(I)> | 7.4 | 1.3 |
| Completeness [%] | 99.8 | 98.9 |
| Redundancy | 3.4 | 3.5 |
| CC(1/2) | 0.761 | 0.422 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1 M Citric acid pH 3.5, 25% w/v Polyethylene glycol 3,350 |
