9DS3

Crystal structure of Apo-241_2F04 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	C 1 2 1
Unit cell lengths	151.950, 117.973, 93.428
Unit cell angles	90.00, 120.10, 90.00

Refinement procedure

Resolution	37.670 - 1.900
R-factor	0.2085
Rwork	0.207
R-free	0.23440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.884
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.670	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.170	0.900
Rpim	0.070	0.540
Number of reflections	102332	102180
<I/σ(I)>	8
Completeness [%]	94.0
Redundancy	6.3
CC(1/2)	1.000	0.300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M sodium acetate, pH 6.2, 20% PEG3350