9DS1

Crystal structure of 241_2F04 Fab in complex with H1 HA from A/California/04/2009(H1N1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97934
Spacegroup name	H 3 2
Unit cell lengths	129.466, 129.466, 443.769
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.430 - 2.400
R-factor	0.2222
Rwork	0.220
R-free	0.26330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.644
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.21rc1_5127: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.430	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.180	1.100
Rpim	0.040	0.280
Number of reflections	54268	54123
<I/σ(I)>	15
Completeness [%]	96.0
Redundancy	19
CC(1/2)	1.000	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M sodium acetate, pH 6.4, 16% PEG3350