9DRZ

Structure of Lichtheimia corymbifera Kinase in complex with GDP and MG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97936
Spacegroup name	P 65 2 2
Unit cell lengths	120.469, 120.469, 166.733
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.460 - 2.700
R-factor	0.2239
Rwork	0.221
R-free	0.27989
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.320
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.460	2.830
High resolution limit [Å]	2.700	2.700
Rmeas	0.414
Rpim	0.107	0.954
Number of reflections	20318	2635
<I/σ(I)>	7.6
Completeness [%]	100.0
Redundancy	14.3	15
CC(1/2)	0.993	0.616

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	0.1 M HEPES pH 7.5, 40% v/v Polyethylene glycol 400