9DRU
Crystal structure of 04709_4F04 Fab in complex with H1 HA from A/California/04/2009(H1N1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 115.404, 115.404, 206.330 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.980 - 2.750 |
| R-factor | 0.2145 |
| Rwork | 0.212 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.607 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21rc1_5127: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 2.600 | |
| Rpim | 0.600 | |
| Number of reflections | 41068 | 41066 |
| <I/σ(I)> | 46.4 | |
| Completeness [%] | 98.0 | |
| Redundancy | 20 | |
| CC(1/2) | 1.000 | 0.560 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M sodium acetate, pH 6.4, 18% PEG3350 |
