9DRQ

Mumps virus fusion glycoprotein F stabilized in prefusion conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2018-12-14
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97
Spacegroup name	H 3 2
Unit cell lengths	74.823, 74.823, 460.854
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.410 - 2.160
R-factor	0.2091
Rwork	0.207
R-free	0.24570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.868
Data reduction software	XDS
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.160	4.650	2.160
Rmerge	0.134
Rmeas	0.139
Rpim	0.035
Number of reflections	27169	2881	2687
<I/σ(I)>	18
Completeness [%]	99.8
Redundancy	16.6
CC(1/2)		0.996	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M imidazole pH 8.0, 35% PEG1000, 0.2 M calcium acetate